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Researchers

Enzymology and Molecular Interactions

Enzymology is a branch of biochemistry aiming to understand how enzymes work through the relationship between structure and function and how they fold into their native state.

The core of enzymology consists of the development of reliable activity assays, expression and purification, kinetic characterization, and an structural characterization, which may include determination of subunit structure, molecular mass, cofactor requirement, and post-translational modifications, and molecular inetractions.

A rigorous interactions between biomolecules and pharmaceutical products

We have a long experience in the field of enzymology and in the techniques useful for studying the chemical (mass spectrometry), conformational (fluorescence, circular dichroism, dynamic light scattering), structural (HDX-MS, native-MS), and molecular recognition properties (SPR, ITC, DSC) of macromolecular system.

The combination of these powerful biochemical and biophysical methods can help to rigorously characterize interactions between biomolecules and pharmaceutical products.

Services

DSF’s Enzymology and Molecular Interactions services includes:

  1. Fluorescence analysis methods: Lanthascreen; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP, etc.
  2. Spectroscopic method: Absorption (UV and Vis), fluorescence, circular dichroism, (Near-UV and Far-UV), dynamic light scattering
  3. Microcalorimetry: isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC)
  4. Mass spectrometry (MS): Native-MS, Hydrogen Deuterium Exchange (HDX) MS
  5. Non-mark selection method: SPR (Biacore)
  6. Enzyme Kinetics: determination of enzyme kinetic constants under steady-state and rapid conditions, determination of enzyme inhibition constants

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