Position
Assistant Professor (RTD-B) of Biochemistry
Contacts
Phone: +39 0498275703
e-mail: laura.acquasaliente@unipd.it
Expertise
- Protein Chemistry Techniques: solid-phase peptide synthesis; chemical modification of proteins.
- Analytical Techniques: protein purification; chromatographic and electrophoretic techniques; mass spectrometry (LC-MS/MS); identification of post-translational modifications by peptide mass fingerprint analysis.
- Spectroscopic Techniques: UV-Vis absorption, circular dichroism, and fluorescence spectroscopy; Dynamic Light Scattering (DLS); basic Small-Angle X-ray scattering (SAXS)
- Molecular Recognition: Surface Plasmon Resonance (SPR); Isothermal Titration Calorimetry (ITC); Differential Scanning Calorimetry (DSC)
- Enzyme Kinetics: determination of enzyme kinetic constants under steady-state and rapid conditions (stopped flow); determination of enzyme inhibition constants.
- Molecular Biology techniques: protein expression in mammalian cells, yeast and E.coli; plasmin-DNA purification; site-directed mutagenesis.
- Practical Haemostasis Techniques: determination of clinical coagulation parameters (TT, PT, aPTT, SCT); fibrin generation; platelet agglutination by turbidimetric and impedance aggregometry; multimer size distribution of von Willebrand Factor by agarose gel-electrophoresis.
- Bioinformatics: data analysis software (Origin 8.0, SigmaPlot); interrogation of databases for protein identification and characterization (EXPASY, MASCOT); molecular visualization and modelling software (ViewerPro, PyMol, HyperChem, Chimera, MOE); molecular docking software (HEX, ClusPro); protein structure analysis software (BLUUES, APBS).
- Protein Structure Techniques: robot-automated crystallization; micro-scale crystallization techniques (hanging drop and sitting drop); basic knowledge of NMR (Nuclear Magnetic Resonance); HDX Mass Spectrometry.
